GWNIC Correlative Light and Electron Microscopy Workshop

The George Washington University (GW) Nanofabrication and Imaging Center (GWNIC) presents a five-day correlative microscopy workshop.

Correlative Microscopy Sample

 

Program

This five-day workshop will cover:

  • how to handle complex modern microscopes with multiple modalities;
  • data fusion, including management of data and large files;
  • preparation of samples for light and electron microscopy;
  • real-world workflow including live cell, fixed cell, and resin section imaging; and
  • state-of-the-art instrumentation and new developments coming to market.

The main theme of this workshop is correlating large-area images of the same sample created with both light and high-resolution electron microscopy.

The workshop will be geared towards microscopists who are interested in learning new techniques for special projects or who are in the market for new instruments, particularly neuroscientists or anatomists who are interested in utilizing large area imaging in their research.

Numerous vendors will demonstrate their instruments.

Additional Details

Monday, September 24th through Friday, September 28, 2018; 9:00 am-5:00 pm each day.

All five days of this workshop will be held on GW’s Foggy Bottom campus in the Science and Engineering Hall.

Lunches and coffee breaks will be provided for all participants.

Attendance will be capped at 20 participants.

There is a $750 registration fee to attend this workshop.

Participating Vendors

  • Arivis AG
  • Bitplane (Imaris)
  • Carl Zeiss Microscopy
  • Leica Microsystems, Inc
  • Nikon Instruments, Inc.
  • Olympus Corporation
  • Thermo Fisher Scientific

Questions?

Email [email protected] for more information or to be notified when registration is available.

Monday, September 24, 2018

  • 9:00 am - Welcome
  • 9:15 am - Introduction to correlative light electron microscopy (CLEM) workflow
  • 10:00 am - Fluorescent proteins
  • 10:45 am - Break
  • 11:00 am - Introduction to confocal microscopy
  • 12:00 pm - Lunch
  • 1:30 pm - Sample preparation for electron microscopy LATS
  • 2:00 pm - Introduction to electron microscopy imaging in SEM to look like TEM
  • 2:45 pm - Break
  • 3:15 pm - Big data
  • 4:00 pm - Cutting-edge 3D workflows for high-content, high-resolution, high-flexibility multi-modal microscopy

Tuesday, September 25, 2018

Sessions focus on widefield and confocal light microscopy imaging.

Wednesday, September 26, 2018

Sessions focus on widefield and confocal light microscopy imaging.

Thursday, September 27, 2018

Sessions focus on electron microscopy.

Friday, September 28, 2018

Sessions focus on image analysis with various software packages.

Optional 90-minute lunch sessions by various vendors will introduce participants to different platforms for doing CLEM.

Workshop Location

All five days of the workshop will be held on GW's Foggy Bottom Campus in Science and Engineering Hall (800 22nd St NW, Washington, DC 20052).

Metro

Science and Engineering Hall is one block from the Foggy Bottom/GW DC Metro stop (Orange, Blue and Silver lines).

Maps and schedules are available on the Washington Metropolitan Area Transit Authority (WMATA) website.

Parking

Visitor parking is available in the University Parking Garage (under Science and Engineering Hall). The parking garage entrance is accessible via H Street between 22nd and 23rd Streets.

View more information about fees and other campus parking options.

Lodging

Local accommodations near the George Washington University

Options labeled "On Campus" or "Foggy Bottom" will be closest to the workshop location.