The George Washington University (GW) Nanofabrication and Imaging Center (GWNIC) features state-of-the-art microscopy instrumentation and a newly-constructed Class 100 cleanroom. GWNIC provides university-wide core infrastructure for research in engineering, chemistry, physics, biology, public health, medicine and biomedical sciences. Located at the heart of GW’s Foggy Bottom Campus, the GWNIC is a catalyst for cross-disciplinary collaboration.
GWNIC features ~5,000 sq ft of Class 100 cleanroom with major instrument clusters for lithography, deposition, etching, measurement and characterization.
GWNIC comprises two imaging suites (~5,500 sq ft) with the latest light, confocal and electron microscopes. Sample preparation equipment and services are also available.
Segmentation performed on FIB-SEM images from corneas. Several individual axons are assigned different colors. One axon (aqua) moves in a different direction compared to the rest of the axons in the cluster shown. From Parlanti, P. et al., (2020), Axonal debris accumulates in corneal epithelial cells after intraepithelial corneal nerves are damaged: A focused Ion Beam Scanning Electron Microscopy (FIB-SEM) study in Experimental Eye Research 194 (2020) 107998.
Segmentation performed on FIB-SEM images from corneas. Intraepithelial Corneal Basal Nerves (ICBNs) are assigned the color yellow and the corneal epithelial basement membrane is assigned the color purple. From Parlanti, P. et al., (2020), Axonal debris accumulates in corneal epithelial cells after intraepithelial corneal nerves are damaged: A focused Ion Beam Scanning Electron Microscopy (FIB-SEM) study in Experimental Eye Research 194 (2020) 107998.
Li, H., Uittenbogaard, M., Hao, L., & Chiaramello, A. (2021) Clinical Insights into Mitochondrial Neurodevelopmental and Neurodegenerative Disorders: Their Biosignatures from Mass Spectrometry-Based Metabolomics. Metabolites. 2021 Apr 10;11(4):233. https://doi.org/10.3390/metabo11040233
Vacher, C. M., Lacaille, H., O'Reilly, J. J., Salzbank, J., Bakalar, D., Sebaoui, S., Liere, P., Clarkson-Paredes, C., Sasaki, T., Sathyanesan, A., Kratimenos, P., Ellegood, J., Lerch, J. P., Imamura, Y., Popratiloff, A., Hashimoto-Torii, K., Gallo, V., Schumacher, M., & Penn, A. A. (2021) Placental endocrine function shapes cerebellar development and social behavior. Nat Neurosci. 2021 Aug 16. https://doi.org/10.1038/s41593-021-00896-4
Parlanti, P., Pal-Ghosh, S., Williams, A., Tadvalkar, G., Popratiloff, A., & Stepp, M. A. (2020). Axonal debris accumulates in corneal epithelial cells after intraepithelial corneal nerves are damaged: A focused Ion Beam Scanning Electron Microscopy (FIB-SEM) study. Exp. Eye Research, May;194:107998. https://doi.org/10.1016/j.exer.2020.107998
Wang, X., Popratiloff, A., Motahari, Z., LaMantia, A. S., & Mendelowitz, D. (2020) Disrupted Coordination of Hypoglossal Motor Control in a Mouse Model of Pediatric Dysphagia in DiGeorge/22q11.2 Deletion Syndrome. eNeuro. 2020 Oct 9;7(5):ENEURO.0520-19.2020. https://doi.org/10.1523/ENEURO.0520-19.2020
