Confocal Microscopy

The George Washington University (GW) Nanofabrication and Imaging Center (GWNIC) features three confocal microscopes and a multiphoton confocal microscope.

Carl Zeiss 510 Filter-based Confocal Microscope

Zeiss 510

The Zeiss LSM 510 is a fully automated confocal system with live-cell and tissue imaging capacity. This laser scanning microscope features a scanning stage for complex multi-point dynamic time-lapse experiments and high-throughput data collection. 

  • Zeiss Observer Z1 inverted microscope with a comprehensive set of objectives and four lasers for excitation at 7 different wavelengths
    • Objectives; 10x / 0.35, 20x/0.8, 40x/1.3, 63x/1.4, 100x/1.46
    • Laser launch: 405, 458, 488, 514, 561, 633
  • Three photomultipliers on the backward pathway, one on the forward path
  • Fully programmable Zeiss scanning stage for tilting and multi-position
  • Carl Zeiss CO2/temperature control on stage incubator
  • Zen software, Windows 7 operating system, 20GB RAM with a separate 2TB hard disk for data
     

Carl Zeiss 710 Spectral Confocal Microscope

Zeiss LSM 710

The Zeiss LSM 710 laser scanning confocal microscope can be used for long-term whole live-embryo, tissue slices and cell imaging at high resolution. This upright microscope with x/y/z scanning stage, controlled via Zen software to execute complex multipoint temporal acquisition patterns, is supported by custom-designed on-stage incubation. This allows for long-term observations of explanted tissues and control of the temperature and environment either via perfusion or CO2 control. 

  • 32-channel spectral detector
  • Two single channel photomultipliers and a photomultiplier on the forward pathway
  • Six laser lines for excitation (458, 488, 514, 561 & 633 nm plus additional 405 nm diode laser)
  • A customized high-end computer for large data sets (50GB).
  • Objectives: W Plan-Apo 20x/1.0 DIC VIS-IR WD=1.8 water and 25x/0.8 multi-immersion LD LCI Plan-Apochromat (WD=0.55); Plan-Apochromat 10x/0.45 D=2.0; Plan-Apochromat 20x/0.8 M27 D=0.55; Plan- Apochromat 63x/1.40 Oil DIC; Alpha Plan Apochromat 100x/1.46
     

Carl Zeiss Cell Observer Spinning Disk Confocal Microscope

Zeiss Spinning Disc Confocal Microscope

A Zeiss Cell Observer Spinning Disk Confocal microscope is fully integrated for live-imaging at high resolution. It allows for complex time-lapse imaging experiments to be programmed and executed in high throughput manner.

  • Two Photometrics Delta (64 fps, at 512x512) EM CCD cameras, for simultaneous acquisition of two channels
  • Environmental control for CO2 and hypoxia
  • A combination of high numerical aperture (i.e., 100x/1.46) and multi-immersion corrected objective lenses (i.e., 25x/0.8, 560 μ working distance)
  • Virtual slides using high resolution/high magnification objectives (i.e., 100x/1.46 and 150x/1.35) and a color CCD camera
  • Definitive focus
  • Deconvolution, physiological analyses and stitching applications
     

Leica TCS SP8 Multiphoton Confocal Microscope

Leica MultiPhoton Confocal MicroscopeThe Leica TCS SP8 Multiphoton Flexible Supply Unit features White LASER and 2-photon excitation. The White Light Laser is a fully tunable supercontinuum laser with up to eight simultaneously usable lines in the range of 470 – 670 nm for maximal spectral flexibility in combination with AOBS and SP detector. Recording of two dimensional excitationemission spectra turns the system into a spatially resolved fluorescence spectrophotometer for characterization. The White Light Laser offers pulsed excitatation for FLIM with Pulsepicker for adjustable pulse intervals in FLIM. 

This multiphoton microscope with high power femtosecond laser allows for deep tissue imaging. Precompensation for optimization of pulse width is fully integrated in LAS AF for easy control. It is equipped with Leica HyDRLD nondescanned detector. Its supersensitive photon detection with extraordinary quantum efficiency visualizes even faint details from deep tissue sections. The TCS SP8 SMD FLIM system combines the single photon counting technology FLIM with flexible confocal imaging. The system series integrates hardware and software from PicoQuant with the highend confocal system Leica TCS SP8 MP. 

  • Upright Stand DM6000 CFS flex
  • Objective nosepiece H, 6pos,
  • DM 6000 CFS stand Optical Outfit EL6000 with Transmitted Light Brightfield Detector
  • SMD Software Package FLIM, 2 HyD SMD, FOV scanner SP8
  • Switchable beam expander
  • Scanhead: Two HyDs SMD, three Internal PMTs
  • Laser 405 nm AOTF Flexible
  • Laser Kit WLL2 + Pulse Picker
  • IR Laser Chameleon Vision II, 680-1080nm