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NANOFABRICATION AND IMAGING CENTER

 

Carl Zeiss 710 Spectral Confocal Microscope

Zeiss LSM 710

The Zeiss LSM 710 laser scanning confocal microscope can be used for long-term whole live-embryo, tissue slices and cell imaging at high resolution. This upright microscope with x/y/z scanning stage, controlled via Zen software to execute complex multipoint temporal acquisition patterns, is supported by custom-designed on-stage incubation. This allows for long-term observations of explanted tissues and control of the temperature and environment either via perfusion or CO2 control. 

  • 32-channel spectral detector
  • Two single channel photomultipliers and a photomultiplier on the forward pathway
  • Six laser lines for excitation (458, 488, 514, 561 & 633 nm plus additional 405 nm diode laser)
  • A customized high-end computer for large data sets (50GB).
  • Objectives: W Plan-Apo 20x/1.0 DIC VIS-IR WD=1.8 water and 25x/0.8 multi-immersion LD LCI Plan-Apochromat (WD=0.55); Plan-Apochromat 10x/0.45 D=2.0; Plan-Apochromat 20x/0.8 M27 D=0.55; Plan- Apochromat 63x/1.40 Oil DIC; Alpha Plan Apochromat 100x/1.46